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OBJECTIVE:To investigate the effects of Ossotide tabletcombined with Xianling gubao capsule on the postoperative recovery effect oftrans-traumatic-verterbrae screw-setting combined with bone grafting in the treatment of osteoporotic thoracolum-bar vertebral fractures. METHODS:122 patients with osteoporotic thoracolumbar vertebral fractures who were treated with trans-traumatic-verterbrae screw-setting combined with bone grafting were randomly divided into control group and observation group.The two groups were treated by trans-traumatic-verterbrae screw-setting with bone grafting.Based on this,the control group was treated with 0.3-0.6 g Ossotide tablet,three times a day,taken orally after meals;observation group was additionally treated with 1.5 g Xianling gubao capsule,twice a day,taken orally after meals. Both of the two groups took medicine on the day of oper-ation. A course induded 4 weeks,and it lasted 4 courses. The indexes were observed after 3 and 6 months treatment. The changes of pain score,bone mineral density(BMD)levels,bone gla protein(BGP),bone alkali phsphatase(BALP),procollagen I ntermi-nal peptide (PINP) and other bone metabolism biochemical indexes in 2 groups before and after treatment were observed.Mean-while,the clinical efficacy,rates of thoracolumbar vertebral refractures and incidence of adverse reactions in the 2 groups were re-corded.RESULTS:After treatment,the total effective rate in observation group was significantly higher than control group,pain scores in 2 groups were significantly lower than before,and general decreased by time,observation group was lower than control group;BMD,BGP,BALP and PINP levels in 2 groups were significantly higher than before,and general increased by time,ob-servation group was higher than control group;after 6 months,BMD,BALP and PINP levels in control group were significantly higher than before,the differences were statistically significant(P0.05). CONCLUSIONS:Ossotide tablet com-bined with Xianling gubao capsule can significantly improve the bone metabolism biochemical indexes of patients,promote bone mineral density and fracture healing,with good safety in short-term medication.
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BACKGROUND:Articular cartilage regeneration can be regulatedbyautocrineorparacrinesecretionof various cytokines. OBJECTIVE:To analyze biological properties of bone morphogenetic proteins and basic fibroblast growth factor in biological materials for repair of articular cartilage defect. METHODS:Forty New Zealand white rabbits were used and equaly randomized intofourgroups: fibrin, basic fibroblast growth factor, bone morphogenetic protein, and combined treatment (basic fibroblast growth factor combined with bone morphogenetic protein) groups, respectively.Bioactivescaffolds with fibrin, basic fibroblast growth factor,bone morphogenetic protein, and basic fibroblast growth factor combined with bone morphogenetic protein were injected to repair the articular cartilage defect. Therapeutic effect andbiological properties of biological materials were compared. RESULTS AND CONCLUSION:(1) Inthefibrin group,tworabbits appearedto havelimps. Inthebasic fibroblast growth factor group hand functionwaslimited inonerabbit. Inthebone morphogenetic protein group, one had a limpandonewasin a limitation of activity. Inthecombined treatment group,rabbitsrecovered wel andshowedno differencesintheknee joint before and aftersurgery (P< 0.05). (2) General observation: Inthe combined treatment group, soft solution cartilage defects disappeared,andangiogenesis and cartilage were similar with normal tissues. Inthebone morphogenetic protein group, fractured cartilage marginal existedand could not beclosely integrated with normal cartilage. The presence of chondrocytesin the periphery of the defectwas seen under light microscope. Inthefibrin group, defect site and surrounding tissues healed. Inthe basic fibroblast growth factor group, defect was repaired, but not smooth. (3) Results ofhematoxylin and eosin staining: Inthefibrin group, the bone defect was not repaired, obvious depression surface was seen. In basic fibroblast growth factor group, repair of cartilage defect was obvious. There were a lot ofchondrocytes. Inthe bone morphogenetic protein group, the bone defect was repaired; chondrocytes appeared, but irregular arrangement. Inthecombined treatment group, good bone defect repair and a large number of cartilage cels were seen. Taken together, biological materials with fibrin and basic fibroblast growth factor are ideal for repair of articular cartilage defect by promoting formation of cartilage by bone morphogenetic protein and enhancing chondrocyte proliferation by basic fibroblast growth factor.
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<p><b>OBJECTIVE</b>To detect the expression of pan-neuronal marker protein gene product (PGP)9.5 and its clinicopathologic significance in breast cancer.</p><p><b>METHODS</b>The expression of PGP9.5 was examined by immunohistochemistry EnVision method in 196 cases during 2007 to 2011, including 20 normal tissues, 14 cases of fibroadenoma, 18 cases of ductal carcinoma in situ (DCIS) and 144 cases of invasive ductal carcinoma (IDC) of the breast. The relationship between PGP9.5 expression and clinicopathologic characteristics of IDC was assessed.</p><p><b>RESULTS</b>PGP9.5 expression was localized in the stroma of all normal breast tissues, but there was no expression observed in all fibroadenomas and DCIS. Overall, the expression rate of PGP9.5 in IDC was 61.8% (89/144). PGP9.5 expression increased from grade 1 tumors (29.4%, 10/34) to grade 2-3 tumors (71.8%, 79/110; P = 0.000). In addition, patients with less than 3 years disease-free survival tended to show higher PGP9.5 expression (64.8%, 35/54), compared to patients with equal to and/or more than 3 years disease-free survival (46.7%, 42/90; P = 0.035). However, there was no correlation between PGP9.5 expression and tumor size, tumor stage, lymph metastasis, hormone receptor expression.</p><p><b>CONCLUSION</b>PGP9.5 expression is correlated with tumor grade and prognosis in IDC of the breast.</p>
Subject(s)
Adult , Aged , Female , Humans , Middle Aged , Biomarkers, Tumor , Metabolism , Breast Neoplasms , Metabolism , Pathology , Carcinoma, Ductal, Breast , Metabolism , Pathology , Carcinoma, Intraductal, Noninfiltrating , Metabolism , Pathology , Disease-Free Survival , Fibroadenoma , Metabolism , Pathology , Neoplasm Grading , Ubiquitin Thiolesterase , MetabolismABSTRACT
Objective To investigate the combined inhibition effect and the potential mechanism of rapamycin (mammalian target of rapamycin inhibitor) and PD98059 (mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) inhibitor) on mouse colorectal cancer (CRC). Methods S-ICR mice were subcutaneously injected with 20 mg/kg of 1,2-dimethylhydrazine dihydrochloride in the nape for 20 weeks to induce CRC. From the 16th week, the mice were treated with alone or combined injection with 0.25 mg/kg rapamycin and 2.5 mg/kg PD98059. The drugs were administered for 8 weeks. Subsequently, the animals were sacrificed and dissected, the tumor sizes were measured, and the tumors were harvested for pathological assay. Furthermore, the phosphorylation of mTOR, p70S6K, and 4E-BPl proteins was detected by using immunohistoehemistry. Results The mice treated with rapamycin (44. 44 %) or PD 98059 (either alone (38.89%) or combination treatment (6.67%) were significantly less likely to develop cancer compared with mice receiving none of them (77.78%, P<0. 05). The average size of tumors was (6.15±2. 192), (8.85±3. 983), (2.917±0. 191), (16.36±6.855) mm3 respectively (P<0.05).The anti-cancer effect of the combination treatment was substantially significant. The proteins of phospho-mTOR, phospho-p70s6K and phospho-4E-BPl were significantly down-regulated after treatments (all P values < ,0.05). Conclusions Combined treatment was more effective than single-drug treatments of rapamycin or PD98059 alone for the prevention and treatment of mouse CRC. The mTOR signal pathway might be involved in the inhibitory mechanism.